simultaneous spectrophotometric determination of mycophenolate mofetil and its active metabolite in human plasma using chemometrics methods

نویسندگان

tayyebeh madrakian

masoumeh madadi-shad

mohammad soleimani

چکیده

a spectrophotometric method for selective complexation reaction and simultaneous determination of mycophenolate mofetil (mpm), and mycophenolic acid (mpa) using three multivariate chemometric methods, i.e. partial least squares regression, principal component regression and principal component artificial neural networks, is proposed. the method is based on the complexation reaction of mpm and mpa with fe(iii) ion in the solution. a nonionic surfactant, triton x-100, was used for dissolving the complexes and intensifying the signals. the linear determination ranges for the determination of mpa and mpm were 5.0-215.0 mg l-1, and 10.0-1000.0 mg l-1, respectively. the detection limit for mpa and mpm was obtained as 0.3 mg l-1 and 1.1 mg l-1, respectively. satisfactory results were obtained by the combination of spectrophotometric method and chemometrics techniques. the method was successfully applied to the simultaneous determination of mpm and mpa in serum sample and the results were comparable with hplc method.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods

A spectrophotometric method for selective complexation reaction and simultaneous determination of mycophenolate mofetil (MPM), and mycophenolic acid (MPA) using three multivariate chemometric methods, i.e. partial least squares regression, principal component regression and principal component artificial neural networks, is proposed. The method is based on the complexation reaction of ...

متن کامل

Simultaneous spectrophotometric determination of ampicillin and penicillin in human plasma using multivariate calibration

An analytical methodology based on spectrophotometric and partial least squares (PLS) algorithm for thesimultaneous determination of ampicillin and penicillin in human plasma was developed and validated. Themultivariate model was developed as a binary calibration model and it was built and validated with anindependent set of synthesis and real samples in presence of matrix. It is shown how a de...

متن کامل

Simultaneous Determination of 6-Mercaptopurine and its Oxidative Metabolites in Synthetic Solutions and Human Plasma using Spectrophotometric Multivariate Calibration Methods.

INTRODUCTION 6-Mercaptopurine (6MP) is an important chemotherapeutic drug in the conventional treatment of childhood acute lymphoblastic leukemia (ALL). It is catabolized to 6-thiouric acid (6TUA) through 8-hydroxo-6-mercaptopurine (8OH6MP) or 6-thioxanthine (6TX) intermediates. METHODS High-performance liquid chromatography (HPLC) is usually used to determine the contents of therapeutic drug...

متن کامل

Spectrophotometric Determination of Mycophenolate Mofetil as Its Charge-Transfer Complexes with Two π-Acceptors

Two simple, selective, and rapid spectrophotometric methods are described for the determination of mycophenolate mofetil (MPM) in pure form and in tablets. Both methods are based on charge-transfer complexation reaction of MPM with p-chloranilic acid (p-CA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in dioxane-acetonitrile medium resulting in coloured product measurable at 520 nm (p-CA)...

متن کامل

Preparation of a Major Metabolite of Iguratimod and Simultaneous Assay of Iguratimod and Its Metabolite by HPLC in Rat Plasma

Iguratimod is a new synthetic disease-modifying antirheumatic drug intended to treat patients with rheumatoid arthritis. A new method using recombinant human CYP450s yeast cells containing c-DNA expressed P450s was applied to identify the metabolic pathways of iguratimod and to prepare its metabolite. The metabolite was isolated, and its structure was identified by quadrupole time-of-flight-mas...

متن کامل

Preparation of a Major Metabolite of Iguratimod and Simultaneous Assay of Iguratimod and Its Metabolite by HPLC in Rat Plasma

Iguratimod is a new synthetic disease-modifying antirheumatic drug intended to treat patients with rheumatoid arthritis. A new method using recombinant human CYP450s yeast cells containing c-DNA expressed P450s was applied to identify the metabolic pathways of iguratimod and to prepare its metabolite. The metabolite was isolated, and its structure was identified by quadrupole time-of-flight-mas...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
analytical and bioanalytical chemistry research

ناشر: iranian chemical society

ISSN

دوره 2

شماره 1 2015

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023